{"id":30295,"date":"2025-11-12T14:26:16","date_gmt":"2025-11-12T06:26:16","guid":{"rendered":"https:\/\/aiportek.com\/?p=30295"},"modified":"2025-11-17T14:47:59","modified_gmt":"2025-11-17T06:47:59","slug":"grant-crft-hipsc-cms-cryopreservation","status":"publish","type":"post","link":"https:\/\/aiportek.com\/en\/grant-crft-hipsc-cms-cryopreservation\/","title":{"rendered":"HONGKE Solutions] Grant CRFT Liquid Nitrogen Free Procedural Cryostat: Efficient Cryopreservation and High Survival Rate of hiPSC-CMs"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"30295\" class=\"elementor elementor-30295\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-303a47ec elementor-section-stretched elementor-section-full_width elementor-section-height-min-height elementor-section-content-middle elementor-section-height-default elementor-section-items-middle\" data-id=\"303a47ec\" data-element_type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;,&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-27d5e225\" data-id=\"27d5e225\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4e369ae3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4e369ae3\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-6555484e\" data-id=\"6555484e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-397ef20e elementor-widget elementor-widget-heading\" data-id=\"397ef20e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Hongke's latest articles<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-4b5c0d9b elementor-absolute elementor-widget elementor-widget-heading\" data-id=\"4b5c0d9b\" data-element_type=\"widget\" data-settings=\"{&quot;_position&quot;:&quot;absolute&quot;}\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">HongKe<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6d18033c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6d18033c\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1f96cecf\" data-id=\"1f96cecf\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fd50b9b elementor-widget elementor-widget-text-editor\" data-id=\"fd50b9b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d303089 elementor-widget elementor-widget-text-editor\" data-id=\"d303089\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4b0e7b4e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4b0e7b4e\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-7a4b7cfc\" data-id=\"7a4b7cfc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-79a3214 elementor-widget elementor-widget-heading\" data-id=\"79a3214\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">[HONGKE SOLUTIONS] Ending Low Survival Rates: Highly Efficient Cryopreservation of hiPSC-CMs and Scale-up of Cardiac Research with the Grant CRFT Liquid Nitrogen-Free Procedural Cooling Apparatus<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-226f412 elementor-widget elementor-widget-post-info\" data-id=\"226f412\" data-element_type=\"widget\" data-widget_type=\"post-info.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"elementor-inline-items elementor-icon-list-items elementor-post-info\">\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-2358f4d elementor-inline-item\" itemprop=\"author\">\n\t\t\t\t\t\t<a href=\"https:\/\/aiportek.com\/en\/author\/hongketechnology\/\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg 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elementor-post-info__item--type-author\">\n\t\t\t\t\t\t\t\t\t\tHongKeTechnology\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t<\/li>\n\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-a689c23 elementor-inline-item\" itemprop=\"datePublished\">\n\t\t\t\t\t\t<a href=\"https:\/\/aiportek.com\/en\/2025\/11\/12\/\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-calendar\" viewbox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M12 192h424c6.6 0 12 5.4 12 12v260c0 26.5-21.5 48-48 48H48c-26.5 0-48-21.5-48-48V204c0-6.6 5.4-12 12-12zm436-44v-36c0-26.5-21.5-48-48-48h-48V12c0-6.6-5.4-12-12-12h-40c-6.6 0-12 5.4-12 12v52H160V12c0-6.6-5.4-12-12-12h-40c-6.6 0-12 5.4-12 12v52H48C21.5 64 0 85.5 0 112v36c0 6.6 5.4 12 12 12h424c6.6 0 12-5.4 12-12z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text elementor-post-info__item elementor-post-info__item--type-date\">\n\t\t\t\t\t\t\t\t\t\t<time>November 12, 2025<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t<\/li>\n\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c027dd7 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"c027dd7\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3a8309a elementor-widget elementor-widget-text-editor\" data-id=\"3a8309a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"230\" data-end=\"325\">In Human Heart Disease Modeling and Regenerative Medicine Research.<strong data-start=\"248\" data-end=\"278\">Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)<\/strong> It is seen as a \"critical tool\" with great potential - it can simulate pathologies, screen for new drugs, and even provide a source of cells for heart repair. However<strong data-start=\"330\" data-end=\"355\">Difficulty in standardized production, large batch variation, functional impairment after freezing.<\/strong>The three major bottlenecks of hiPSC-CMs in scientific research and clinical translation have long been identified.<\/p><p data-start=\"391\" data-end=\"588\">Important research recently published by the Kevin Kit Parker Group at Harvard University has shown that by optimizing <strong data-start=\"433\" data-end=\"446\">Stirring Suspension Culture System<\/strong>In this breakthrough study, we successfully realized the efficient and reproducible generation of hiPSC-CMs. In this groundbreaking study, the<strong data-start=\"490\" data-end=\"542\">Grant CRF-1 Liquid Nitrogen Free Programmable Cryostat (now upgraded to Grant CRFT model), distributed by HONGKEI.<\/strong>It plays a central role in the cell cryopreservation process, dramatically improving the stability of experimental results and the potential for large-scale application.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9aba933 elementor-widget elementor-widget-heading\" data-id=\"9aba933\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-small\">01. Why is cryopreservation of hiPSC-CMs so critical?<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c22f34e elementor-widget elementor-widget-image\" data-id=\"c22f34e\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"573\" src=\"https:\/\/aiportek.com\/wp-content\/uploads\/2025\/11\/640-9-1024x573.webp\" class=\"attachment-large size-large wp-image-30297\" alt=\"\" srcset=\"https:\/\/aiportek.com\/wp-content\/uploads\/2025\/11\/640-9-1024x573.webp 1024w, https:\/\/aiportek.com\/wp-content\/uploads\/2025\/11\/640-9-300x168.webp 300w, https:\/\/aiportek.com\/wp-content\/uploads\/2025\/11\/640-9-768x430.webp 768w, https:\/\/aiportek.com\/wp-content\/uploads\/2025\/11\/640-9-18x10.webp 18w, https:\/\/aiportek.com\/wp-content\/uploads\/2025\/11\/640-9-600x336.webp 600w, https:\/\/aiportek.com\/wp-content\/uploads\/2025\/11\/640-9.webp 1080w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d3381e7 elementor-widget elementor-widget-text-editor\" data-id=\"d3381e7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"627\" data-end=\"701\">Conventional monolayer culture of hiPSC-CMs suffers from poor scale-up, uneven nutrient distribution, and large batch-to-batch variation, while the old suspension culture strategy performs poorly in terms of differentiation consistency and cell quality assessment.<\/p><p data-start=\"703\" data-end=\"845\"><strong data-start=\"703\" data-end=\"716\">Bottlenecks in the refrigeration recovery sector<\/strong>The application of hiPSC-CMs is even more restricted. Past studies have shown that the survival rate of monolayer-differentiated hiPSC-CMs after cryopreservation is only about 60%, and the problems of cell shrinkage, abnormal electrophysiological properties, and deviation of drug response are frequently occurring, which makes the \"cryopreservation\" become the core obstacle hindering the large-scale application of hiPSC-CMs.<\/p><p data-start=\"847\" data-end=\"1011\">To address these challenges.<strong data-start=\"855\" data-end=\"873\">Boston Children's Hospital and Harvard University team<\/strong>A novel stirred suspension differentiation system has been developed, which is capable of stably generating ventricular muscle-based hiPSC-CMs with a functional purity of approximately 94%. However, if these cells are to be truly \"usable and usable\", it will be necessary to develop a new system that can be used to generate the cells in the future.<strong data-start=\"942\" data-end=\"959\">Highly efficient and standardized cryo-recovery technology<\/strong>It is indispensable - after all, not every experiment can be \"differentiated and used now\", and a standardized cryopreservation process is a prerequisite for moving towards large-scale research and clinical application.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d254321 elementor-widget elementor-widget-heading\" data-id=\"d254321\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-small\">02. How to realize precise cryopreservation and efficient recovery of hiPSC-CMs?<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f707efe elementor-widget elementor-widget-text-editor\" data-id=\"f707efe\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"1052\" data-end=\"1136\">In the \"cell cryo-recovery\" stage of the study, the research team explicitly used the <strong data-start=\"1081\" data-end=\"1099\">Grant Liquid Nitrogen Free Programmable Cryostat<\/strong>(provided by Hongke), whose core function is the realization of the<strong data-start=\"1115\" data-end=\"1128\">Precise and controlled cooling process<\/strong>The specific steps are as follows:<\/p><p data-start=\"1138\" data-end=\"1211\">1\ufe0f\u20e3 <strong data-start=\"1142\" data-end=\"1150\">Cell Preparation<\/strong><br data-start=\"1150\" data-end=\"1153\" \/>The differentiated and matured hiPSC-CMs were resuspended in a special freezing solution (STEMdiff\u2122 Cardiomyocyte Freezing Medium) and dispensed into cryopreservation tubes.<\/p><p data-start=\"1213\" data-end=\"1293\">2\ufe0f\u20e3 <strong data-start=\"1217\" data-end=\"1226\">Programmatic Cooling<\/strong><br data-start=\"1226\" data-end=\"1229\" \/>The rate of cooling is controlled by Grant's liquid nitrogen-free programmable cryostat, which allows the cryotubes to be cooled steadily down to -80\u00b0C within 1 hour, avoiding rapid cooling that could result in ice crystals that could damage the cells.<\/p><p data-start=\"1295\" data-end=\"1344\">3\ufe0f\u20e3 <strong data-start=\"1299\" data-end=\"1307\">Long-term storage<\/strong><br data-start=\"1307\" data-end=\"1310\" \/>After cooling, transfer the cryotubes to -150\u00b0C liquid nitrogen for long-term storage.<\/p><p data-start=\"1346\" data-end=\"1404\">4\ufe0f\u20e3 <strong data-start=\"1350\" data-end=\"1358\">Subsequent recovery<\/strong><br data-start=\"1358\" data-end=\"1361\" \/>When needed, the tubes can be removed from liquid nitrogen and thawed quickly in a 37\u00b0C water bath before being used for subsequent functional experiments.<\/p><p data-start=\"1406\" data-end=\"1485\">In a nutshell.<strong data-start=\"1411\" data-end=\"1434\">Grant CRFT Liquid Nitrogen Free Programmable Cryostat<\/strong>Our mission is to create a \"gentle, controlled freezing environment\" for hiPSC-CMs that maximizes cell viability and functional integrity.<\/p><p data-start=\"1487\" data-end=\"1569\"><strong data-start=\"1490\" data-end=\"1498\">end result<\/strong>: In the study, the Grant CRFT descaler was used to realize up to <strong data-start=\"1527\" data-end=\"1543\">Cell Resuscitation Survival Rate of 94%<\/strong>The 60% level is significantly better than the 60% level of conventional methods and remains fully functional.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-43de91f elementor-widget elementor-widget-heading\" data-id=\"43de91f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-small\">03. Grant CRFT Liquid Nitrogen Free Programmable Cryostat Core Benefits<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f1928a3 elementor-widget elementor-widget-text-editor\" data-id=\"f1928a3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"1608\" data-end=\"1628\"><strong>\ud83d\udd39 Precise temperature control to avoid 'ice crystal killers'<\/strong><\/p><p data-start=\"1629\" data-end=\"1766\">The greatest risk of cellular freezing is \"ice crystal formation\", which can puncture cell membranes and cause irreversible damage. The Grant CRFT can be programmed to precisely control the rate of cooling (to a steady -80\u00b0C in about 1 hour), allowing cells to gradually dehydrate and avoid ice crystal formation, thus achieving the following <strong data-start=\"1731\" data-end=\"1755\">Resuscitation survival rate of &gt;94% (Taipan Blue Negative Test)<\/strong>It is much better than the traditional freezing method.<\/p><p data-start=\"1773\" data-end=\"1792\"><strong>\ud83d\udd39 Standardize processes to eliminate batch variation<\/strong><\/p><p data-start=\"1793\" data-end=\"1949\">\"Lot-to-lot consistency\" has always been a challenge for the reproducibility of hiPSC-CMs. grant CRFT has been able to achieve this through the use of<strong data-start=\"1841\" data-end=\"1852\">Fully automatic program control<\/strong>This design ensures that the freezing curves are exactly the same each time and avoids human error. This design ensured that as many as 14 iPSC cell lines and 25 differentiation experiments were able to consistently produce high purity cardiomyocytes for<strong data-start=\"1924\" data-end=\"1941\">Research Standardization and Clinical Cell Production<\/strong>Provides the technical basis.<\/p><p data-start=\"1956\" data-end=\"1981\"><strong>\ud83d\udd39 Highly adaptable to support large-volume freezing and multi-scene applications<\/strong><\/p><p data-start=\"1982\" data-end=\"2144\">The research team utilized Grant CRFT to complete a single 380 mL cultivation volume of about <strong data-start=\"2020\" data-end=\"2036\">13.2 Billion sCMs<\/strong> The high efficiency of cryopreservation provides a stable cellular reserve for subsequent engineered cardiac tissue construction and high-throughput drug screening. This excellent<strong data-start=\"2083\" data-end=\"2098\">Capacity Adaptability and Operational Stability<\/strong>This makes the Grant CRFT a key piece of equipment for moving hiPSC-CMs from laboratory research to large-scale production.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d48ced2 elementor-widget elementor-widget-heading\" data-id=\"d48ced2\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-small\">Conclusion<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-005880d elementor-widget elementor-widget-text-editor\" data-id=\"005880d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"2161\" data-end=\"2230\">This study not only proves that<strong data-start=\"2169\" data-end=\"2181\">Stirring Suspension Separation System<\/strong>advantages in the production of hiPSC-CMs, highlighting even more the<strong data-start=\"2204\" data-end=\"2229\">The Core Value of Precision Cryogenic Equipment in Regenerative Medicine Research Transformation<\/strong>The<\/p><p data-start=\"2232\" data-end=\"2299\"><strong data-start=\"2232\" data-end=\"2255\">Grant CRFT Liquid Nitrogen Free Programmable Cryostat<\/strong> With its performance of \"high survival rate, no loss of function and batch consistency\", it has become a powerful assistant for the research team to break through the bottleneck of scale-up.<\/p><p data-start=\"2301\" data-end=\"2429\">For those who focus on<strong data-start=\"2306\" data-end=\"2327\">Stem Cell Research, Heart Disease Modeling and Regenerative Medicine<\/strong>The Grant CRFT is not just a cryostat, but a bridge between laboratory and clinical applications for research institutions - it enables the ready reuse of high-quality hiPSC-CMs, which is a powerful support for disease modeling, drug discovery, and industrialization of cellular therapies.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-45ed7c8 elementor-widget elementor-widget-button\" data-id=\"45ed7c8\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/aiportek.com\/biotech\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Go to the Grant product page<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-9049411 e-flex e-con-boxed e-con e-parent\" data-id=\"9049411\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-cfcf4de e-flex e-con-boxed e-con e-parent\" data-id=\"cfcf4de\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-dbc1b58 elementor-widget elementor-widget-heading\" data-id=\"dbc1b58\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-xl\">Other Articles<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-74adc8f elementor-posts--align-left elementor-grid-3 elementor-grid-tablet-2 elementor-grid-mobile-1 elementor-posts--thumbnail-top elementor-card-shadow-yes elementor-posts__hover-gradient elementor-widget elementor-widget-posts\" data-id=\"74adc8f\" data-element_type=\"widget\" data-settings=\"{&quot;cards_row_gap&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:100,&quot;sizes&quot;:[]},&quot;cards_columns&quot;:&quot;3&quot;,&quot;cards_columns_tablet&quot;:&quot;2&quot;,&quot;cards_columns_mobile&quot;:&quot;1&quot;,&quot;cards_row_gap_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;cards_row_gap_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"posts.cards\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-posts-container elementor-posts elementor-posts--skin-cards elementor-grid\" role=\"list\">\n\t\t\t\t<article class=\"elementor-post elementor-grid-item post-34221 post type-post status-publish format-standard has-post-thumbnail hentry category-18 tag-aimotive tag-64\" role=\"listitem\">\n\t\t\t<div class=\"elementor-post__card\">\n\t\t\t\t<a class=\"elementor-post__thumbnail__link\" href=\"https:\/\/aiportek.com\/en\/hongke-dual-modal-autonomous-driving-simulation-3dgs-aisim\/\" tabindex=\"-1\" target=\"_blank\"><div class=\"elementor-post__thumbnail\"><img decoding=\"async\" width=\"1080\" height=\"587\" src=\"https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1-1.webp\" class=\"attachment-full size-full wp-image-34223\" alt=\"\" srcset=\"https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1-1.webp 1080w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1-1-300x163.webp 300w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1-1-1024x557.webp 1024w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1-1-768x417.webp 768w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1-1-18x10.webp 18w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1-1-600x326.webp 600w\" sizes=\"(max-width: 1080px) 100vw, 1080px\" \/><\/div><\/a>\n\t\t\t\t<div class=\"elementor-post__badge\">Hongke Case<\/div>\n\t\t\t\t<div class=\"elementor-post__text\">\n\t\t\t\t<h3 class=\"elementor-post__title\">\n\t\t\t<a href=\"https:\/\/aiportek.com\/en\/hongke-dual-modal-autonomous-driving-simulation-3dgs-aisim\/\" target=\"&quot;_blank&quot;\">\n\t\t\t\t\u3010\u8679\u79d1\u65b9\u6848\u3011 \u8679\u79d1\u96d9\u6a21\u614b\u4eff\u771f\u6e2c\u8a66\u65b9\u6848\uff1a3DGS \u8207 aiSim \u6df1\u5ea6\u89e3\u6790 | \u6578\u5b57\u5b7f\u751f\t\t\t<\/a>\n\t\t<\/h3>\n\t\t\t\t<div class=\"elementor-post__excerpt\">\n\t\t\t<p>\u8679\u79d1\u63a8\u51fa\u81ea\u52d5\u99d5\u99db\u96d9\u6a21\u614b\u4eff\u771f\u65b9\u6848\uff0c\u7d50\u5408 aiSim \u7269\u7406\u5f15\u64ce\u8207 3DGS\/NeRF \u6280\u8853\uff0c\u5be6\u73fe\u7269\u7406\u7d1a\u50b3\u611f\u5668\u5efa\u6a21\u8207\u9ad8\u4fdd\u771f\u5834\u666f\u91cd\u5efa\uff0c\u89e3\u6c7a\u7aef\u5230\u7aef\u667a\u99d5\u9589\u74b0\u6e2c\u8a66\u96e3\u984c\u3002<\/p>\n\t\t<\/div>\n\t\t\n\t\t<a class=\"elementor-post__read-more\" href=\"https:\/\/aiportek.com\/en\/hongke-dual-modal-autonomous-driving-simulation-3dgs-aisim\/\" aria-label=\"Read more about \u3010\u8679\u79d1\u65b9\u6848\u3011 \u8679\u79d1\u96d9\u6a21\u614b\u4eff\u771f\u6e2c\u8a66\u65b9\u6848\uff1a3DGS \u8207 aiSim \u6df1\u5ea6\u89e3\u6790 | \u6578\u5b57\u5b7f\u751f\" tabindex=\"-1\" target=\"_blank\">\n\t\t\tRead more\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-post__meta-data\">\n\t\t\t\t\t<span class=\"elementor-post-author\">\n\t\t\tHongKeTechnology\t\t<\/span>\n\t\t\t\t<span class=\"elementor-post-date\">\n\t\t\t2026\u5e744\u670814\u65e5\t\t<\/span>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/article>\n\t\t\t\t<article class=\"elementor-post elementor-grid-item post-34207 post type-post status-publish format-standard has-post-thumbnail hentry category-18 tag-vuzix tag-42\" role=\"listitem\">\n\t\t\t<div class=\"elementor-post__card\">\n\t\t\t\t<a class=\"elementor-post__thumbnail__link\" href=\"https:\/\/aiportek.com\/en\/pcb-inspection-ar-ai-workflow\/\" tabindex=\"-1\" target=\"_blank\"><div class=\"elementor-post__thumbnail\"><img decoding=\"async\" width=\"1080\" height=\"721\" src=\"https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640.webp\" class=\"attachment-full size-full wp-image-34213\" alt=\"\" srcset=\"https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640.webp 1080w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-300x200.webp 300w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-1024x684.webp 1024w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-768x513.webp 768w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-18x12.webp 18w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/04\/640-600x401.webp 600w\" sizes=\"(max-width: 1080px) 100vw, 1080px\" \/><\/div><\/a>\n\t\t\t\t<div class=\"elementor-post__badge\">Hongke Case<\/div>\n\t\t\t\t<div class=\"elementor-post__text\">\n\t\t\t\t<h3 class=\"elementor-post__title\">\n\t\t\t<a href=\"https:\/\/aiportek.com\/en\/pcb-inspection-ar-ai-workflow\/\" target=\"&quot;_blank&quot;\">\n\t\t\t\t\u3010\u8679\u79d1\u65b9\u6848\u3011AI \u8b58\u5225\u8207 AR \u5de5\u4f5c\u6d41\uff1a\u91cd\u5851 PCB \u8cea\u6aa2\u826f\u7387\u8207\u6578\u5b57\u5316\u5de5\u5ee0\t\t\t<\/a>\n\t\t<\/h3>\n\t\t\t\t<div class=\"elementor-post__excerpt\">\n\t\t\t<p>\u9762\u81e8 PCB \u8cea\u6aa2\u826f\u7387\u74f6\u9838\uff1f\u8679\u79d1 HongKe \u63d0\u4f9b AR \u6a19\u6e96\u5316\u5de5\u4f5c\u6d41 + AI \u8b58\u5225\u89e3\u6c7a\u65b9\u6848\uff0c\u5c07\u8089\u773c\u6f0f\u6aa2\u7387\u964d\u4f4e 50%\uff0c\u65b0\u54e1\u5de5\u57f9\u8a13\u7e2e\u77ed\u81f3 1 \u5929\u3002\u9069\u7528\u65bc\u9999\u6e2f\u53ca\u6771\u5357\u4e9e\u96fb\u5b50\u88fd\u9020\u8f49\u578b\uff0c\u52a9\u60a8\u5be6\u73fe\u9ad8\u6548\u54c1\u8cea\u63a7\u88fd\u8207\u54c1\u8cea\u8ffd\u6eaf\u3002<\/p>\n\t\t<\/div>\n\t\t\n\t\t<a class=\"elementor-post__read-more\" href=\"https:\/\/aiportek.com\/en\/pcb-inspection-ar-ai-workflow\/\" aria-label=\"Read more about \u3010\u8679\u79d1\u65b9\u6848\u3011AI \u8b58\u5225\u8207 AR \u5de5\u4f5c\u6d41\uff1a\u91cd\u5851 PCB \u8cea\u6aa2\u826f\u7387\u8207\u6578\u5b57\u5316\u5de5\u5ee0\" tabindex=\"-1\" target=\"_blank\">\n\t\t\tRead more\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-post__meta-data\">\n\t\t\t\t\t<span class=\"elementor-post-author\">\n\t\t\tHongKeTechnology\t\t<\/span>\n\t\t\t\t<span class=\"elementor-post-date\">\n\t\t\t2026\u5e744\u67088\u65e5\t\t<\/span>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/article>\n\t\t\t\t<article class=\"elementor-post elementor-grid-item post-34192 post type-post status-publish format-standard has-post-thumbnail hentry category-18 tag-gnss tag-67\" role=\"listitem\">\n\t\t\t<div class=\"elementor-post__card\">\n\t\t\t\t<a class=\"elementor-post__thumbnail__link\" href=\"https:\/\/aiportek.com\/en\/gnss-simulator-gaussian-noise-solution\/\" tabindex=\"-1\" target=\"_blank\"><div class=\"elementor-post__thumbnail\"><img loading=\"lazy\" decoding=\"async\" width=\"1080\" height=\"484\" src=\"https:\/\/aiportek.com\/wp-content\/uploads\/2026\/02\/640-3.png\" class=\"attachment-full size-full wp-image-33152\" alt=\"\" srcset=\"https:\/\/aiportek.com\/wp-content\/uploads\/2026\/02\/640-3.png 1080w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/02\/640-3-300x134.png 300w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/02\/640-3-1024x459.png 1024w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/02\/640-3-768x344.png 768w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/02\/640-3-18x8.png 18w, https:\/\/aiportek.com\/wp-content\/uploads\/2026\/02\/640-3-600x269.png 600w\" sizes=\"(max-width: 1080px) 100vw, 1080px\" \/><\/div><\/a>\n\t\t\t\t<div class=\"elementor-post__badge\">Hongke Case<\/div>\n\t\t\t\t<div class=\"elementor-post__text\">\n\t\t\t\t<h3 class=\"elementor-post__title\">\n\t\t\t<a href=\"https:\/\/aiportek.com\/en\/gnss-simulator-gaussian-noise-solution\/\" target=\"&quot;_blank&quot;\">\n\t\t\t\t\u3010\u8679\u79d1\u65b9\u6848\u3011\u8679\u79d1 GNSS \u6a21\u64ec\u5668\u9ad8\u65af\u566a\u8072\u89e3\u6790 &#8211; \u63d0\u5347\u5b9a\u4f4d\u7cbe\u5ea6\u8207\u63a5\u6536\u6a5f\u6027\u80fd\t\t\t<\/a>\n\t\t<\/h3>\n\t\t\t\t<div class=\"elementor-post__excerpt\">\n\t\t\t<p>\u6df1\u5165\u89e3\u6790\u8679\u79d1 GNSS \u6a21\u64ec\u5668\u4e2d\u9ad8\u65af\u566a\u8072\u5c0d\u5b9a\u4f4d\u6548\u679c\u7684\u5f71\u97ff\u3002\u4e86\u89e3\u5982\u4f55\u900f\u904e\u79d1\u5b78\u6a21\u64ec\u63d0\u5347\u63a5\u6536\u6a5f\u6027\u80fd\u8207\u5b9a\u4f4d\u7cbe\u5ea6\uff0c\u9069\u7528\u65bc\u9999\u6e2f\u53ca\u6771\u5357\u4e9e B2B \u6280\u8853\u6e2c\u8a66\u74b0\u5883\u3002<\/p>\n\t\t<\/div>\n\t\t\n\t\t<a class=\"elementor-post__read-more\" href=\"https:\/\/aiportek.com\/en\/gnss-simulator-gaussian-noise-solution\/\" aria-label=\"Read more about \u3010\u8679\u79d1\u65b9\u6848\u3011\u8679\u79d1 GNSS \u6a21\u64ec\u5668\u9ad8\u65af\u566a\u8072\u89e3\u6790 &#8211; \u63d0\u5347\u5b9a\u4f4d\u7cbe\u5ea6\u8207\u63a5\u6536\u6a5f\u6027\u80fd\" tabindex=\"-1\" target=\"_blank\">\n\t\t\tRead more\t\t<\/a>\n\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-post__meta-data\">\n\t\t\t\t\t<span class=\"elementor-post-author\">\n\t\t\tHongKeTechnology\t\t<\/span>\n\t\t\t\t<span class=\"elementor-post-date\">\n\t\t\t2026\u5e743\u670831\u65e5\t\t<\/span>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/article>\n\t\t\t\t<\/div>\n\t\t\n\t\t\t\t<div class=\"e-load-more-anchor\" data-page=\"1\" data-max-page=\"29\" data-next-page=\"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295\/page\/2\/\"><\/div>\n\t\t\t\t<nav class=\"elementor-pagination\" aria-label=\"Pagination\">\n\t\t\t<span class=\"page-numbers prev\">\"<\/span>\n<span aria-current=\"page\" class=\"page-numbers current\"><span class=\"elementor-screen-only\">Page<\/span>1<\/span>\n<a class=\"page-numbers\" href=\"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295\/page\/2\/\"><span class=\"elementor-screen-only\">Page<\/span>2<\/a>\n<a class=\"page-numbers\" href=\"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295\/page\/3\/\"><span class=\"elementor-screen-only\">Page<\/span>3<\/a>\n<span class=\"page-numbers dots\">...<\/span>\n<a class=\"page-numbers\" href=\"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295\/page\/5\/\"><span class=\"elementor-screen-only\">Page<\/span>5<\/a>\n<a class=\"page-numbers next\" href=\"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295\/page\/2\/\">\"<\/a>\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Harvard study confirms that hiPSC-CMs can survive cryo-recovery up to 94% with no loss of functionality and batch consistency using stirred suspension culture + Grant CRFT liquid nitrogen free programmed cooling. The solution covers STEMdiff\u2122 cryopreservation medium, programmed temperature reduction to -80\u00b0C, -150\u00b0C long-term storage and 37\u00b0C rapid recovery, with complete introduction and technical support provided by HONGKEI.<\/p>","protected":false},"author":1,"featured_media":30297,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[18],"tags":[63,45],"class_list":["post-30295","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-18","tag-grant","tag-45"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/comments?post=30295"}],"version-history":[{"count":6,"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295\/revisions"}],"predecessor-version":[{"id":30304,"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/posts\/30295\/revisions\/30304"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/media\/30297"}],"wp:attachment":[{"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/media?parent=30295"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/categories?post=30295"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/aiportek.com\/en\/wp-json\/wp\/v2\/tags?post=30295"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}